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Isolering og karakterisering af Cardiac mesenchymale stromale celler fra Endomyocardial Bioptic prøver af Arrhythmogenic kardiomyopati patienter
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
DOI:

09:16 min

February 28, 2018

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Chapters

  • 00:04Title
  • 01:12Cardiac Biopsy Collection and Processing
  • 03:06Characterizing Cardiac Mesenchymal Stromal Cells (C-MSC) by Flow Cytometry
  • 05:22Oil Red O (ORO) Staining C-MSC after Adipogenic Differentiation
  • 07:02Results: Flow Cytometry Analysis and ORO Staining
  • 08:27Conclusion

Summary

Automatic Translation

I denne artikel findes en metode til at isolere hjerte mesenchymale stromale celler fra endomyocardial bioptic prøver af arrhythmogenic kardiomyopati patienter. Deres karakterisering og protokollen til at øge deres adipogenic differentiering er beskrevet.

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