Journal
/
Neuroscience
/
新生小鼠脑巴细胞基因调控的立体定向手术
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
Please note that all translations are automatically generated. Click here for the English version.

新生小鼠脑巴细胞基因调控的立体定向手术

DOI:
10.3791/57270-v

•

09:44 min

•

July 10, 2018

•

, ,
1Institute of Neuroscience,National Yang-Ming University, 2Brain Research Center,National Yang-Ming University

Chapters

  • 00:04Title
  • 00:40Preparing the Microinjection Apparatus
  • 04:09Microinjecting Neonatal Mice
  • 07:30Results: Analysis of Injection of Cre-expressing Virus into P2 Striatum
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个立体定向手术的协议, 一个自制的头固定装置 microinjecting 试剂进入新生鼠脑纹状体。这种技术允许在新生小鼠大脑特定区域的神经细胞中进行基因操作。

Tags

Stereotaxic SurgeryGenetic ManipulationStriatal CellsNeonatal Mouse BrainsPostnatal DevelopmentVisual DemonstrationHome-made DeviceTargeted InjectionsHead TrayStainless Steel NeedlesChloroformEthanolMicroinjection AssemblyPE20 Polyethylene TubingPE10 Tubing26 Gauge Syringe30 Gauge Injection Needle10 Microliter Microinjection Syringe25 Gauge Syringe0.1% Fast Green DyeFiltered Solution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

啮齿动物的立体定向手术和动物福利成果改进行为神经科学

小鼠多巴胺和三维胶原基质检测纹状体外植体的解剖和文化

特定脑区的兴奋毒性损伤的成年大鼠立体定向手术治疗

可视化和遗传操作在小鼠大脑皮层和使用海马树突和棘<em>在子宫内</em>电穿孔

立体定向注射病毒载体的基因操作在小鼠脊髓条件

遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔

整装免疫荧光染色新生小鼠视网膜血管生成调查<em>在体内</em

小脑颗粒神经元的遗传操作<em>体外</em>和<em>在体内</em>为研究神经元形态和迁移

光学相干断层扫描: 成像小鼠视网膜神经节细胞在体内

在普通马莫塞特中植入微电极阵列的立体外科（Callithrix jacchus）

Read Article