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新生小鼠脑巴细胞基因调控的立体定向手术
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
DOI:

09:44 min

July 10, 2018

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Chapters

  • 00:04Title
  • 00:40Preparing the Microinjection Apparatus
  • 04:09Microinjecting Neonatal Mice
  • 07:30Results: Analysis of Injection of Cre-expressing Virus into P2 Striatum
  • 09:03Conclusion

Summary

Automatic Translation

我们描述了一个立体定向手术的协议, 一个自制的头固定装置 microinjecting 试剂进入新生鼠脑纹状体。这种技术允许在新生小鼠大脑特定区域的神经细胞中进行基因操作。

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