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启动子捕获高分辨率, 全基因组的启动子交互分析
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
DOI:

10:16 min

June 28, 2018

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Chapters

  • 00:04Title
  • 00:52Formaldehyde Fixation
  • 02:14HindIII Digestion and Biotinylation of Restriction Fragment Overhangs
  • 04:09Hybrid In-solution Capture
  • 06:53Isolation of Promoter Fragment-containing Ligation Products
  • 08:55Results: IL8 PCHi-C Profile in Human Hematopoietic Cells
  • 09:34Conclusion

Summary

Automatic Translation

DNA 调控元素, 例如促进剂, 通过物理接触靶基因促进剂控制基因表达, 通常通过长距离染色体相互作用跨越大的基因组距离。启动子捕获高 c (PCHi) 确定促进者和远端区域之间的重要相互作用, 使潜在的调控序列分配给他们的目标基因。

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