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1Nuclear Dynamics Programme, The Babraham Institute,Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol,Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute,Babraham Research Campus, 5Department of Biological Science,Florida State University
Chapters
- 00:04Title
- 00:52Formaldehyde Fixation
- 02:14HindIII Digestion and Biotinylation of Restriction Fragment Overhangs
- 04:09Hybrid In-solution Capture
- 06:53Isolation of Promoter Fragment-containing Ligation Products
- 08:55Results: IL8 PCHi-C Profile in Human Hematopoietic Cells
- 09:34Conclusion
DNA regulerende elementer, såsom smagsforstærkere, kontrollerer genekspression ved fysisk at kontakte target gen initiativtagere, ofte gennem langtrækkende kromosom vekselvirkninger der spænder over stor genomisk afstande. Promotor fange Hi-C (PCHi-C) identificerer betydningsfulde vekselvirkninger mellem projektledere og distale regioner, gør det muligt for tildelingen af potentielle regulerende sekvenser til deres mål gener.
