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Journal
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Immunology and Infection
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小鼠胰岛细胞筛选致糖尿病环境化学物质的研究
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Immunology and Infection
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JoVE Journal
Immunology and Infection
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
Please note that all translations are automatically generated.
Click here for the English version.
小鼠胰岛细胞筛选致糖尿病环境化学物质的研究
DOI:
10.3791/57327-v
•
07:39 min
•
June 25, 2018
•
Jingshu Chen*
2
,
Lei Zhong*
1
,
Jing Wu
,
Sui Ke
,
Benjamin Morpurgo
,
Andrei Golovko
,
Nengtai Ouyang
,
Yuxiang Sun
,
Shaodong Guo
,
Yanan Tian
2
1
Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization
,
Hunan Agriculture University
,
2
Texas A&M University
,
3
Texas A&M Institute for Genomic Medicine
,
4
Sun Yat-Sen Memorial Hospital
Chapters
00:04
Title
00:49
Pancreas Perfusion and Removal
01:43
Pancreas Digestion and Purification of Islets
03:30
Plating and Treatment of Islet Cells
04:23
ROS Staining and Measurement
05:28
Results: Xenobiotic Treatment-induced Reactive Oxygen Species and Inhibition of Insulin Secretion in Isolated Pancreatic Islet Cells
06:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 以隔离小鼠胰岛细胞筛选的 ROS 归纳的外来化合物, 以确定潜在的致糖尿病 xenobiotic 化学品。
Tags
Pancreas
Islet Cells
ROS
Type-2 Diabetes
In Vitro
Collagenase
Digestion
Isolation
Purification
Glucose-stimulated Insulin Secretion
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