/
/
Optogenetic de arrastre de las oscilaciones Theta Hippocampal en comportarse ratones
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Systems Neurophysiology Research Group, Institute of Clinical Neuroscience and Medical Psychology, Medical Faculty,Heinrich Heine University Düsseldorf, 2Behavioural Neurodynamics Group,Leibniz Institute for Molecular Pharmacology (FMP)/ NeuroCure Cluster of Excellence, 3Neuronal Circuits and Behavior Research Group,Max Planck Institute for Metabolism Research
Chapters
- 00:04Title
- 00:39Viral Injection
- 01:52Preparation of Optic Fibers
- 02:45Preparation of Tungsten Wire Arrays for LFP Recordings
- 03:56Stereotaxic Implantation
- 05:26Optogenetic Stimulation and Electrophysiological Data Acquisition
- 06:15Results: Optogenetic Entrainment of Theta Rhythm
- 07:02Conclusion
Describimos el uso de optogenetics y grabaciones electrofisiológicas para la manipulación selectiva de oscilaciones de hippocampal de la theta (5-10 Hz) en ratones de comportarse. La eficacia del arrastre de ritmo se controla usando el potencial de campo local. Una combinación de opto - e inhibición farmacogenética aborda la lectura eferente del hipocampo sincronización.
