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跟踪果蝇幼虫行为对嗅神经元 Optogenetic 刺激的反应
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Neuroscience
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JoVE Journal Neuroscience
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
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跟踪果蝇幼虫行为对嗅神经元 Optogenetic 刺激的反应

DOI:
10.3791/57353-v

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06:49 min

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March 21, 2018

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, , , , , ,
1Department of Biology, MS-0314,University of Nevada, 2Integrated Neuroscience Graduate Program,University of Nevada

Chapters

  • 00:04Title
  • 00:38Building Behavioral Arena and Preparing Optogenetic Stimulation Hardware
  • 02:49Preparing Drosophila Larvae Behavior Analyses
  • 04:36Behavior Assay
  • 05:42Results: Specific ORN Activation
  • 06:17Conclusion

Summary

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Automatic Translation

该协议分析了果蝇幼虫对其嗅觉神经元同时 optogenetic 刺激的导航行为。光的 630 nm 波长是用来激活单个嗅神经元表达一个红色转移通道紫红。幼虫运动是同时跟踪, 数字记录, 并分析使用自定义编写的软件。

Tags

Drosophila LarvaOptogenetic StimulationOlfactory NeuronsBehavior ArenaCCD CameraInfrared LEDLED PlatformRaspberry PiCsChrimsonORX-GAL4

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