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Neuroscience

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JoVE Journal Neuroscience
Seguimiento de comportamiento larvario de Drosophila en respuesta a la estimulación de la Optogenetic de las neuronas olfatorias
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Seguimiento de comportamiento larvario de Drosophila en respuesta a la estimulación de la Optogenetic de las neuronas olfatorias

Article DOI: 10.3791/57353
March 21st, 2018 •
1,2, 1, 1, 1, 1, 1,2, 1,2
1Department of Biology, MS-0314, University of Nevada, 2Integrated Neuroscience Graduate Program, University of Nevada

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March 21st, 2018

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Este protocolo analiza comportamiento navegación de larva de Drosophila en respuesta a la estimulación simultánea de la optogenetic de sus neuronas olfativas. Luz de longitud de onda de 630 nm se usa para activar las neuronas olfativas individuales expresando una rodopsina canal rojo-cambiado de puesto. Movimiento de larvas al mismo tiempo se realiza un seguimiento grabado digitalmente y analizados utilizando el software de medida.

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