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Neuroscience

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JoVE Journal Neuroscience
Sporing Drosophila larve adfærd som svar på Optogenetic Stimulation af olfaktoriske neuroner
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Sporing Drosophila larve adfærd som svar på Optogenetic Stimulation af olfaktoriske neuroner

Article DOI: 10.3791/57353
March 21st, 2018 •
1,2, 1, 1, 1, 1, 1,2, 1,2
1Department of Biology, MS-0314, University of Nevada, 2Integrated Neuroscience Graduate Program, University of Nevada

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March 21st, 2018

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Denne protokol analyserer navigations opførsel af Drosophila larve i svar til samtidige optogenetic stimulation af dens olfaktoriske neuroner. Lys af 630 nm bølgelængde bruges til at aktivere individuelle olfaktoriske neuroner giver udtryk for en rød-skiftede kanal rhodopsin. Larve bevægelse spores samtidig, registreret digitalt og analyseret ved hjælp af brugerdefinerede-skrevet software.

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