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Biology
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RNA 干渉法の活用: ゴキブリのリポソーム キャリアで二本鎖 RNA の経口デリバリー
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JoVE Journal
Biology
Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
Please note that all translations are automatically generated.
Click here for the English version.
RNA 干渉法の活用: ゴキブリのリポソーム キャリアで二本鎖 RNA の経口デリバリー
DOI:
10.3791/57385-v
•
08:26 min
•
May 01, 2018
•
Jia-Hsin Huang
,
Yun Liu
,
Yu-Hsien Lin
2,3
,
Xavier Belles
,
How-Jing Lee
1
Department of Entomology
,
National Taiwan University
,
2
Biology Centre, Institute of Entomology
,
Czech Academy of Sciences
,
3
Faculty of Science
,
University of South Bohemia
,
4
Institute of Evolutionary Biology, CSIC-UPF
Chapters
00:04
Title
00:50
Collection of Extracellular Enzymes from Hemolymph
02:13
Collection of Extracellular Enzymes from Midgut Juice
03:31
dsRNA Degradation Assay
05:31
Oral Administration of dsRNA
06:42
Results: Knock Down of Tubulin Expression in the Midgut
07:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
この原稿は、リポソームにカプセル化された二本鎖 RNA の経口摂取によってドイツのゴキブリの中腸における遺伝子発現の減少を示しています。
Tags
RNA Interference
Double-stranded RNA
Liposome Carriers
Cockroaches
Pest Control
Gene Silencing
Hemolymph Collection
Midgut Juice
Insect Saline Buffer
Protein Quantification
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