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Bioengineering
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优化液体搬运机器人对
拟南芥
进行高通量前化学遗传学筛选的应用
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of
Arabidopsis thaliana
Please note that all translations are automatically generated.
Click here for the English version.
优化液体搬运机器人对
拟南芥
进行高通量前化学遗传学筛选的应用
DOI:
10.3791/57393-v
•
11:58 min
•
April 30, 2018
•
B. K. Amos
,
Victoria G. Pook
,
Seth Debolt
1
Department of Horticulture
,
University of Kentucky
Chapters
00:05
Title
00:56
Creating a Dilution Library
05:03
Adding Media-seed Mixture to Screening Plates
06:40
Adding Small Molecules to Screening Plates
10:16
Results: Representative Results from a Forward Chemical Genetics Screen on Arabidopsis
11:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在模型植物物种 (
拟南芥
) 上, 对合成小分子进行了高通量筛查。该协议是为液体处理机器人开发的, 它提高了正向化学遗传学筛的速度, 加速了新的小分子对植物生理学的发现。
Tags
Liquid Handling Robot
High Throughput
Forward Chemical Genetics Screen
Arabidopsis Thaliana
Small Molecule Screening
Automation
Dilution Library
96-well Plates
Pipette Tips
Liquid Handling
Dilution
Chemical Library
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