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Journal / Cancer Research / Isolamento de células endoteliais humanas do…
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Cancer Research

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JoVE Journal Cancer Research
Isolamento de células endoteliais humanas do cólon Normal e Carcinoma colorrectal - um protocolo melhorado
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Isolamento de células endoteliais humanas do cólon Normal e Carcinoma colorrectal - um protocolo melhorado

Article DOI: 10.3791/57400-v • 10:54 min • April 4th, 2018
April 4th, 2018 •
1, 1, 1, 2, 3, 4, 4, 2, *2, *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
* These authors contributed equally

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Células endoteliais de tumor são importantes determinantes do microambiente do tumor e o curso da doença. Aqui, um protocolo para o isolamento de células endoteliais puras e viáveis de carcinoma colorectal humana e cólon normal para ser usado em pesquisa de teste e patogênese da droga é descrito.

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Pesquisa sobre o câncer edição 134 células endoteliais navio Carcinoma colorretal Tumor cólon Normal CD31 VE-caderina CD105 FACS-classificação tecido dissociação microambiente do Tumor fibroblastos
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