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Biology
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磁気活性化細胞を分離し、滑膜液由来間葉系幹細胞が家兎モデルからの浄化戦略を並べ替え
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Biology
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JoVE Journal
Biology
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
Please note that all translations are automatically generated.
Click here for the English version.
磁気活性化細胞を分離し、滑膜液由来間葉系幹細胞が家兎モデルからの浄化戦略を並べ替え
DOI:
10.3791/57466-v
•
10:20 min
•
August 10, 2018
•
Zhaofeng Jia
2,3
,
Yujie Liang
5
,
Xingfu Li
3
,
Xiao Xu
2,3
,
Jianyi Xiong
3
,
Daping Wang
2,3
,
Li Duan
3
1
Postgraduate institution
,
Guangzhou Medical University
,
2
Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology
,
3
Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopaedic Engineering
,
Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University)
,
4
Department of Chemistry
,
Chinese University of Hong Kong
,
5
Shenzhen Kangning Hospital
,
Shenzhen Mental Health Center
Chapters
00:04
Title
01:07
Isolate and Culture the rbSF-MSCs
03:21
CD90-positive Magnetic Activated Cell Sorting (MACS) of the rbSF-MSCs and Primary Culture
05:01
Magnetic Separation and Culture of te CD90
+
rbSF-MSCs
06:44
Results: Obtain Purified Rabbit SF-MSCs by CD90-Based Magnetic Activated Cell Sorting
08:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
この記事では、簡単な分離そしてニュージーランド白ウサギ滑液からの間葉系幹細胞の浄化のためのシンプルで経済的プロトコルを示します。
Tags
Magnetic-activated Cell Sorting
Mesenchymal Stem Cells
Synovial Fluid
Rabbit Model
Isolation
Purification
FDA-approved
Cell Culture
Cell Suspension
Centrifugation
Cell Strainer
Cell Colonies
Cell Passaging
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