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Journal
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Bioengineering
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MicroRNA 移植成人骨髓造血干细胞促进细胞工程与磁性靶向结合的协议
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Bioengineering
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JoVE Journal
Bioengineering
Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
Please note that all translations are automatically generated.
Click here for the English version.
MicroRNA 移植成人骨髓造血干细胞促进细胞工程与磁性靶向结合的协议
DOI:
10.3791/57474-v
•
11:37 min
•
June 18, 2018
•
Frauke Hausburg*
1,2
,
Paula Müller*
1,2
,
Natalia Voronina*
1
,
Gustav Steinhoff
2
,
Robert David
2
1
Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery
,
Rostock University Medical Center
,
2
Department Life, Light and Matter of the Interdisciplinary Faculty
,
Rostock University
Chapters
00:04
Title
00:53
CD133 SC Isolation
06:00
Characterization of Freshly Isolated CD133 SCs
07:21
Preparation of Transfection Complexes
08:26
CD133 SCs Transfection and Analysis
10:03
Results: Successful Engineering of miR-loaded Magnetized CD133 Cells
11:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议说明了一个安全和有效的程序, 以修改 CD133
+
造血干细胞。所提出的非病毒, 磁性 polyplex 公司的方法可以为优化治疗干细胞效应, 并通过磁共振成像监测所管理的细胞产品提供了依据。
Tags
MicroRNA Transfer
Adult Bone Marrow-derived Hematopoietic Stem Cells
Cell Engineering
Magnetic Targeting
Regenerative Medicine
Hematopoietic Stem Cell Modification
Mesenchymal Stem Cells
Bone Marrow Collection
Density Gradient Centrifugation
Mononuclear Cell Isolation
Magnetic Selection
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