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Developmental Biology
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CARIP 和芯片序列: 鉴定胚胎干细胞中染色质相关 rna 和蛋白质-DNA 相互作用的方法
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Developmental Biology
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JoVE Journal
Developmental Biology
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
CARIP 和芯片序列: 鉴定胚胎干细胞中染色质相关 rna 和蛋白质-DNA 相互作用的方法
DOI:
10.3791/57481-v
•
11:13 min
•
May 25, 2018
•
Benjamin L. Kidder
2
1
Department of Oncology
,
Wayne State University School of Medicine
,
2
Karmanos Cancer Institute
,
Wayne State University School of Medicine
Chapters
00:04
Title
01:02
Crosslinking of Embryonic Stem Cells for ChIP and CARIP
02:29
Chromatin Sonication for ChIP and CARIP
03:48
ChIP and CARIP Process
07:12
Double-stranded cDNA Synthesis for CARIP-Seq
08:53
Use of ChIP-Seq and CARIP-Seq to Study Global Protein-DNA Interactions and Chromatin-Associated RNAs in Embryonic Stem Cells
10:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了执行芯片序列和 CARIP 的方法, 包括用于下一代测序的库准备, 以生成 ES 细胞中的全球 epigenomic 和染色质相关的 RNA 图谱。
Tags
CARIP-Seq
ChIP-Seq
Chromatin-associated RNAs
Protein-DNA Interactions
Embryonic Stem Cells
Epigenetics
Transcription Factors
Epigenetic Regulators
Histone Modifications
Chromatin Immunoprecipitation
Sonication
Formaldehyde Cross-linking
Glycine
Phosphate Buffered Saline
Trypsinization
MEF Medium
Fetal Bovine Serum
Sonication Buffer
Sodium Dodecyl Sulfate
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