Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFR&#945;+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Xiaomin Dong; Raquel Cuevas-Diaz Duran; Yanan You; Jia Qian Wu

doi:10.3791/57547

Journal

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Neuroscience

/

低细胞染色质免疫沉淀测序分析 Olig2 基因组结合位点在急性纯化 PDGFRα + 细胞中的转录因子鉴定

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

低细胞染色质免疫沉淀测序分析 Olig2 基因组结合位点在急性纯化 PDGFRα + 细胞中的转录因子鉴定

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9,183 Views

•

12:29 min

•

April 16, 2018

DOI:

10.3791/57547-v

DOI:

10.3791/57547-v

•

12:29 min

April 16, 2018

•

14 Views

Xiaomin Dong¹, Raquel Cuevas-Diaz Duran¹, Yanan You¹, Jia Qian Wu¹

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine,University of Texas Health Science Center

Transcript

Summary

在这里, 我们提出一个协议, 旨在分析少突胶质转录因子 2 (Olig2) 在急性纯化脑少突胶质前体细胞 (OPCs) 的全基因组结合执行低细胞染色质免疫沉淀 (芯片)、库准备、高通量测序和生物信息学数据分析。

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低细胞染色质免疫沉淀测序分析 Olig2 基因组结合位点在急性纯化 PDGFRα + 细胞中的转录因子鉴定

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