低细胞染色质免疫沉淀测序分析 Olig2 基因组结合位点在急性纯化 PDGFRα + 细胞中的转录因子鉴定

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JoVE Journal Neuroscience

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

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低细胞染色质免疫沉淀测序分析 Olig2 基因组结合位点在急性纯化 PDGFRα + 细胞中的转录因子鉴定

DOI:

10.3791/57547-v

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12:29 min

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April 16, 2018

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Xiaomin Dong, Raquel Cuevas-Diaz Duran, Yanan You, Jia Qian Wu

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine,University of Texas Health Science Center

Chapters

00:04Title
01:10Purification of PDGFRα Positive Oligodendrocyte Lineage Cells from Mouse
05:03Low-cell ChIP Preparation and ChIP Library Construction for High-throughput Sequencing
09:41Results: Use of ChIP Analysis to Study Genome-wide Binding of the Olig2 in OPCs
11:42Conclusion

Summary

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在这里, 我们提出一个协议, 旨在分析少突胶质转录因子 2 (Olig2) 在急性纯化脑少突胶质前体细胞 (OPCs) 的全基因组结合执行低细胞染色质免疫沉淀 (芯片)、库准备、高通量测序和生物信息学数据分析。

低细胞染色质免疫沉淀测序分析 Olig2 基因组结合位点在急性纯化 PDGFRα + 细胞中的转录因子鉴定

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