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Journal / Neuroscience / PDGFRα + 셀 낮은 셀 Chromatin Immunoprecipitation 시퀀싱 …
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Neuroscience

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JoVE Journal Neuroscience
PDGFRα + 셀 낮은 셀 Chromatin Immunoprecipitation 시퀀싱 분석에 의해 정화에 녹음 방송 요인 Olig2 게놈 바인딩 사이트를 심하게 식별
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PDGFRα + 셀 낮은 셀 Chromatin Immunoprecipitation 시퀀싱 분석에 의해 정화에 녹음 방송 요인 Olig2 게놈 바인딩 사이트를 심하게 식별

Article DOI: 10.3791/57547-v • 12:29 min • April 16th, 2018
April 16th, 2018 •
1, 1, 1, 1
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

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여기에 우리가 현재 낮은 셀 chromatin immunoprecipitation (칩)를 수행 하 여 분석 하는 게놈 넓은의 바인딩을 oligodendrocyte 녹음 방송 요인 2 (Olig2) 심하게 정화 뇌 oligodendrocyte 선구자 세포 (OPCs) 하도록 설계 된 프로토콜 도서관 준비, 높은 처리량 시퀀싱 및 bioinformatic 데이터 분석.

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신경 과학 문제점 134 OPCs Olig2 낮은 셀 칩 seq transcriptional 규칙 immunopanning MACS2
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