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JoVE Journal Neuroscience
At identificere transskription faktor Olig2 genomisk bindingssteder i akut renset PDGFRα + celler ved lav-celle kromatin Immunoprecipitation sekventering analyse
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Journal (Neuroscience)
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Journal (Neuroscience)
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At identificere transskription faktor Olig2 genomisk bindingssteder i akut renset PDGFRα + celler ved lav-celle kromatin Immunoprecipitation sekventering analyse

Article DOI: 10.3791/57547-v • 12:29 min • April 16th, 2018
April 16th, 2018 •
1, 1, 1, 1
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

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Her præsenterer vi en protokol, der er designet til at analysere genome-wide bindingen af oligodendrocyte transskription faktoren 2 (Olig2) i akut renset hjernen oligodendrocyte forløber celler (OPCs) ved at udføre lav-celle kromatin immunoprecipitation (ChIP) , bibliotek forberedelse, høj overførselshastighed sekvensering og bioinformatic dataanalyse.

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Neurovidenskab spørgsmål 134 OPCs Olig2 lav-celle ChIP-FF. transkriptionel regulering immunopanning MACS2
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