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Chapters
- 00:00Title
- 01:11Sample preparation and contaminant exclusion
- 02:10Homogenization and DNA extraction
- 04:41Sequencing library construction
- 05:48Purification of the PCR reaction
- 06:19DNA quality check and quantification, and sequencing
- 07:43Sequencing of the DNA library
- 08:02Representative results
- 09:35Closing remarks
Forurensing under den genomisk sekvensering av mikroskopiske organismer er fortsatt et stort problem. Her viser vi en metode for å sekvens genomet av en Bjørnedyr fra en enkelt prøve med 50 pg genomisk DNA uten hele genomet forsterkning å minimere risikoen for forurensning.
