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Chapters
- 00:00Title
- 01:11Sample preparation and contaminant exclusion
- 02:10Homogenization and DNA extraction
- 04:41Sequencing library construction
- 05:48Purification of the PCR reaction
- 06:19DNA quality check and quantification, and sequencing
- 07:43Sequencing of the DNA library
- 08:02Representative results
- 09:35Closing remarks
Forurening under den genomisk sekventering af mikroskopiske organismer er fortsat et stort problem. Her viser vi en metode til sequence genomet af et tardigrade fra et enkelt eksemplar med så lidt som 50 pg genomisk DNA uden hele genom forstærkning til at minimere risikoen for kontaminering.
