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1Department of Internal Medicine-Gastroenterology,University of Michigan, 2Department of Molecular and Integrative Physiology,University of Michigan, 3Department of Molecular, Cellular and Developmental Biology,University of Michigan, 4Department of Gastrointestinal Surgery,The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology,University of Arizona College of Medicine
Chapters
- 00:04Title
- 00:33Dissection of the Intestinal Segment
- 03:01Removal of the Epithelial Mucosa
- 04:57Collection of Enteric Glial Cells from the Lamina Propria and Submucosa
- 06:23Results: Enteric Glial Cells 24 h After Plating
- 07:12Conclusion
Här beskriver vi isolering av enteriska-glial celler från intestinal-submucosa använda sekventiell EDTA inkubationer för att kelat divalenta katjoner och sedan inkubation i icke-enzymatiska cell återställningslösning. Plätering resulterande cellsuspension på poly-D-lysin och laminin resulterar i en höganrikat kultur av submukösa gliaceller för funktionell analys.
