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Romanen leveringsmåten plasmider DNA til musen blæren Urothelium av Electroporation
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JoVE Journal Biology
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Romanen leveringsmåten plasmider DNA til musen blæren Urothelium av Electroporation

DOI:
10.3791/57649-v

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06:38 min

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May 03, 2018

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, , ,
1Department of Molecular, Cell, and Developmental Biology,University of California, Santa Cruz

Chapters

  • 00:05Title
  • 01:06Urine Depletion and Bladder Rinsing
  • 02:40Plasmid Delivery
  • 05:16Results: Successful Delivery of pCAG-GFP Plasmid to the Mouse Bladder Urothelium
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en ny metode for levering av DNA plasmider inn i urothelial celler av musen blæren i vivo gjennom urinrøret catheterization og electroporation. Det tilbyr en rask og enkel måte for autochtonous musen modeller av blæren sykdommer.

Tags

Plasmid DNA DeliveryBladder UrotheliumElectroporationGene OverexpressionGenome EditingBladder CancerGene TherapyAnesthesiaCatheterPBS WashingDNA PlasmidTrypan BlueVertical Incision

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