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Biology
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用自动光学相机进行细胞迁移的
体外
试验优化划痕试验
JoVE Journal
Biology
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JoVE Journal
Biology
Optimized Scratch Assay for
In Vitro
Testing of Cell Migration with an Automated Optical Camera
Please note that all translations are automatically generated.
Click here for the English version.
用自动光学相机进行细胞迁移的
体外
试验优化划痕试验
DOI:
10.3791/57691-v
•
05:27 min
•
August 08, 2018
•
Michelle Vang Mouritzen
,
Håvard Jenssen
1
Department of Science and Environment
,
Roskilde University
Chapters
00:04
Title
00:23
HaCaT Monolayer Generation
01:50
Migration Stimulation and Optical Camera Setup
03:59
Results: Representative Wound Healing Analyses
04:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了一个程序, 以测试的细胞迁移
体外
与自动光学相机显微镜。刮伤试验已被广泛使用, 在结束后的划痕是一个设定的周期。光学显微镜能够可靠、廉价地检测细胞的迁移。
Tags
Cell Migration
Scratch Assay
Automated Optical Camera
HaCaT Cells
Trypsin
Mitomycin C
Wound Healing
Cell Proliferation
Endothelial Growth Factor
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