/

/

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

JoVE Journal
Genetics

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Genetics

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

DOI:

10.3791/57731-v

•

09:30 min

•

September 13, 2018

•

Clark Fritsch², Jean-Francois Pierre Gout⁴, Marc Vermulst

¹Center for Mitochondrial and Epigenomic Medicine,Children’s Hospital of Philadelphia, ²Department of Cellular and Molecular Biology,University of Pennsylvania, ³Department of Biological Sciences,Mississippi State University, ⁴Center for Mechanisms of Evolution, Biodesign Institute,Arizona State University

Chapters

00:04Title
00:38RNA Circularization and Rolling Circle Reverse Transcription
02:51Second Strand cDNA Synthesis and Repair
03:45Adapter Ligation and Size Selection
06:21PCR Amplification and Final Bead Purification
07:53Results: Quality Control, Bio-Informatic Analysis, and Expected Results from the C-seq Assay
08:54Conclusion

Summary

Automatic Translation

This protocol provides researchers with a new tool to monitor the fidelity of transcription in multiple model organisms.

Tags

Keywords: Transcription Errors Eukaryotic Organisms Transcriptional Mutagenesis RNA Polymerase Bioinformatics Circularize RNA T4 RNA Ligase Reverse Transcription Second Strand Synthesis Genome-wide Surveillance

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

In Vitro Transcription Assays and Their Application in Drug Discovery

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Generation of Genome-wide Chromatin Conformation Capture Libraries from Tightly Staged Early Drosophila Embryos

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Read Article