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Journal
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Genetics
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真核生物の転写エラーのゲノム全体の監視
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Genetics
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JoVE Journal
Genetics
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
Please note that all translations are automatically generated.
Click here for the English version.
真核生物の転写エラーのゲノム全体の監視
DOI:
10.3791/57731-v
•
09:30 min
•
September 13, 2018
•
Clark Fritsch
2
,
Jean-Francois Pierre Gout
4
,
Marc Vermulst
1
Center for Mitochondrial and Epigenomic Medicine
,
Children’s Hospital of Philadelphia
,
2
Department of Cellular and Molecular Biology
,
University of Pennsylvania
,
3
Department of Biological Sciences
,
Mississippi State University
,
4
Center for Mechanisms of Evolution, Biodesign Institute
,
Arizona State University
Chapters
00:04
Title
00:38
RNA Circularization and Rolling Circle Reverse Transcription
02:51
Second Strand cDNA Synthesis and Repair
03:45
Adapter Ligation and Size Selection
06:21
PCR Amplification and Final Bead Purification
07:53
Results: Quality Control, Bio-Informatic Analysis, and Expected Results from the C-seq Assay
08:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、複数のモデル生物における転写の忠実度を監視する新しいツールと研究者を提供します。
Tags
Transcription Errors
Eukaryotic Organisms
Transcriptional Mutagenesis
RNA Polymerase
Bioinformatics
Circularize RNA
T4 RNA Ligase
Reverse Transcription
Second Strand Synthesis
Genome-wide Surveillance
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