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大鼠外周全血细胞免疫表型和因子生成的单细胞分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
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大鼠外周全血细胞免疫表型和因子生成的单细胞分析

DOI:
10.3791/57780-v

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12:36 min

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June 26, 2018

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, , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2OMNI Biomarkers, Development Sciences,Genentech, 3Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Chapters

  • 00:04Title
  • 01:10Stimulation and Processing of Peripheral Whole Blood
  • 05:27Barcode of Lysed/Fixed Blood Cells
  • 09:44Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
  • 11:50Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个单细胞蛋白质组学方法, 以评估免疫表型和功能 (细胞因子诱导) 改变周围全血标本, 分析通过大规模细胞术。

Tags

Single-cell AnalysisImmunophenotypeCytokine ProductionPeripheral Whole BloodMass CytometryAutoimmunityCell FrequencyCytokine ProductionSystems ImmunologyAutoimmune DiseaseSLEWhole Blood ProcessingRuxolitinibLyse/fix BufferCell RecoveryAntibody Staining

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