JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
光谱分析 TRPV1 的纯化与重构
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
Please note that all translations are automatically generated.
Click here for the English version.
光谱分析 TRPV1 的纯化与重构
DOI:
10.3791/57796-v
•
11:53 min
•
July 03, 2018
•
Francisco J. Sierra-Valdez
,
Richard A. Stein
,
Phanindra Velissety
3
,
Valeria Vasquez
,
Julio F. Cordero-Morales
1
Department of Physiology
,
University of Tennessee Health Science Center
,
2
Department of Molecular Physiology and Biophysics
,
Vanderbilt University Medical Center
,
3
CuriRX, Inc.
Chapters
00:04
Title
00:54
TRPV1 Mutagenesis
02:19
Generating the Recombinant Bacmid and Baculovirus for Protein Expression
05:14
eTRPV1 and Single-cysteine Mutant Purification
07:18
eTRPV1 Single-cysteine Mutant Site-directed Spin Labeling
08:12
eTRPV1 Spin-labeled Single Cysteine Mutant Reconstitution
09:30
Results: Characterization of the Minimal Cysteine-less TRPV1 Construct and Single-cysteine Mutants used for Spectroscopic Analysis
11:08
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了获得洗涤剂-可溶性 TRPV1 的生化量的具体方法, 用于光谱分析。这些联合协议提供了生物化学和生物物理工具, 可用于促进膜控制环境中哺乳动物离子通道的结构和功能研究。
Tags
TRPV1
Ion Channel
Spectroscopic Analysis
Electron Paramagnetic Resonance
Double Electron Electron Resonance
Conformational Changes
Thermal Gating
Light-independent Gating
PCR Mutagenesis
Recombinant Bacmid
ETRPV1
Cysteine Mutants
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
化学感受器配体结合域的高效复用与纯化方法
2在 1: 一步亲和纯化为蛋白质蛋白质和蛋白质代谢物的平行的分析
台式固定化金属亲和层析、Polyhistidine 标记金属酶的重组与检测
体外
支持性脂质双层自组织蛋白模式的重组
氧电极对 DesB 酶活性和抑制作用的厌氧蛋白纯化及动力学分析
富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
活性
秀丽隐杆线
虫核提取物的分离及重建用于
体外
转录
用完全纯化的组分重构Msp1提取活性
Purification of Endogenous
Drosophila
Transient Receptor Potential Channels
从猪肾和小鼠肝脏中提取和纯化FAHD1蛋白
Read Article