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Immunology and Infection
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人网膜和皮下脂肪组织 CD34+CD31+ 内皮细胞的分离、扩增和脂肪诱导
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Please note that all translations are automatically generated.
Click here for the English version.
人网膜和皮下脂肪组织 CD34+CD31+ 内皮细胞的分离、扩增和脂肪诱导
DOI:
10.3791/57804-v
•
10:28 min
•
July 17, 2018
•
Bronson A. Haynes
,
Ryan W. Huyck
,
Ashley J. James
,
Meghan E. Carter
,
Omnia U. Gaafar
,
Marjorie Day
,
Avennette Pinto
,
Anca D. Dobrian
1
Department of Physiological Sciences
,
Eastern Virginia Medical School
Chapters
00:04
Title
01:01
Adipose Stromal Vascular Fraction Isolation
03:07
Isolation of Adipose Tissue Endothelial Cells
06:08
Induction of Adipogenesis in Isolated Endothelial Cells
07:13
Results: CD31+CD34+ have Endothelial Phenotype and Display Depot- and Subject-specific Responses to Adipogenic Induction
08:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
脂肪组织血管祖细胞中白、米色脂肪体的分化具有改善肥胖代谢的潜力。我们描述了 CD34+CD31+ 内皮细胞与人的脂肪分离的协议, 并为随后
的体外
扩增和分化为白色和米色脂肪。讨论了几种下游应用。
Tags
CD34+CD31+ Endothelial Cells
Adipogenesis
Obesity
Cell Isolation
Collagenase Digestion
Stromal Vascular Fraction
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