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Isolation, ekspansion og Adipogenic induktion af CD34 + CD31 + endotelceller fra menneskelige Omental og subkutane fedtvæv
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
DOI:

10:28 min

July 17, 2018

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Chapters

  • 00:04Title
  • 01:01Adipose Stromal Vascular Fraction Isolation
  • 03:07Isolation of Adipose Tissue Endothelial Cells
  • 06:08Induction of Adipogenesis in Isolated Endothelial Cells
  • 07:13Results: CD31+CD34+ have Endothelial Phenotype and Display Depot- and Subject-specific Responses to Adipogenic Induction
  • 08:57Conclusion

Summary

Automatic Translation

Differentiering af hvide og beige adipocytter fra fedtvæv vaskulære stamfaderen bjørne potentiale for metaboliske forbedring i fedme. Vi beskriver protokoller for en CD34 + CD31 + endotel celle isolation fra menneskelige fedt og en efterfølgende in vitro- udvidelse og differentiering af hvide og beige adipocytter. Flere downstream ansøgninger behandles.

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