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人网膜和皮下脂肪组织 CD34+CD31+ 内皮细胞的分离、扩增和脂肪诱导
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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人网膜和皮下脂肪组织 CD34+CD31+ 内皮细胞的分离、扩增和脂肪诱导

DOI:
10.3791/57804-v

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10:28 min

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July 17, 2018

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, , , , , , ,
1Department of Physiological Sciences,Eastern Virginia Medical School

Chapters

  • 00:04Title
  • 01:01Adipose Stromal Vascular Fraction Isolation
  • 03:07Isolation of Adipose Tissue Endothelial Cells
  • 06:08Induction of Adipogenesis in Isolated Endothelial Cells
  • 07:13Results: CD31+CD34+ have Endothelial Phenotype and Display Depot- and Subject-specific Responses to Adipogenic Induction
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

脂肪组织血管祖细胞中白、米色脂肪体的分化具有改善肥胖代谢的潜力。我们描述了 CD34+CD31+ 内皮细胞与人的脂肪分离的协议, 并为随后的体外扩增和分化为白色和米色脂肪。讨论了几种下游应用。

Tags

CD34+CD31+ Endothelial CellsAdipogenesisObesityCell IsolationCollagenase DigestionStromal Vascular Fraction

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