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Korrekturlesing og DNA reparasjon analysen Single nukleotid forlengelse og MALDI-TOF massespektrometri analyse
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1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University
Chapters
- 00:05Title
- 01:17Proofreading Reactions
- 03:16Terminate the Reaction and Transfer Reaction Products to a Matrix Chip
- 04:54Setup the Assay Information on the Mass Spectrometry
- 06:18MALDI-TOF Mass Spectrometry (MS) Operation and Data Analysis
- 08:00Results: DNA Proofreading and Repair Analyzed by MALDI-TOF MS
- 09:49Conclusion
En ikke-merket, ikke-radio-isotopanrikning metode for DNA polymerase korrekturlesing og en DNA reparasjon analysen ble utviklet ved hjelp av høy oppløsning MALDI-TOF massespektrometri og en single nukleotid forlengelsen strategi. Analysen viste seg å være svært spesifikke, enkel, rask og lett å utføre for korrekturlesing og reparere patcher kortere enn 9-nukleotider.
