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Chapters
- 00:04Title
- 01:14Gram-negative E. coli Spheroplast Preparation
- 04:12Gram-positive B. megatherium Protoplast Preparation
- 05:58<E. coli Spheroplast and B. megatherium Protoplast Visualization and Anticrobial Peptide (AMP) Localization
- 07:49Results: Representative High-resolution AMP Visualization
- 09:39Conclusion
Here we present a protocol to produce gram-negative Escherichia coli (E. coli) spheroplasts and gram-positive Bacillus megaterium (B. megaterium) protoplasts to clearly visualize and rapidly characterize peptide-bacteria interactions. This provides a systematic method to define membrane localizing and translocating peptides.
