Journal
/
/
Påvisning af Protein subcellulært lokalisering af grøn fluorescens Protein Tagging og 4', 6-Diamidino-2-phenylindole farvning i Caenorhabditis elegans
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4′,6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans
DOI:

09:36 min

July 30, 2018

, ,

Chapters

  • 00:04Title
  • 00:26Heat Stress
  • 02:46Ethanol Fixation and DAPI Staining
  • 04:10Acetone Fixation
  • 05:21Microscopic Imaging
  • 07:56Results: Visualization of C. elegans Exl-1 Subcellular Localization through GFP Tagging and DAPI Staining
  • 09:07Conclusion

Summary

Automatic Translation

Denne protokol demonstrerer, hvordan man spore en protein nukleare translokation under varmestress ved hjælp af en grøn fluorescens protein (NGL) fusion protein som en markør og 4', 6-diamidino-2-phenylindole (DAPI) farvning. DAPI farvning protokol er hurtig og bevarer normal god landbrugspraksis og protein subcellulært lokalisering signaler.

Related Videos

Read Article