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Oppdage Protein Subcellular lokalisering av Green fluorescens Protein merking og 4', 6-Diamidino-2-phenylindole farging i Caenorhabditis elegans
JoVE Journal
Biology
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JoVE Journal Biology
Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4′,6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans
DOI:

09:36 min

July 30, 2018

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Chapters

  • 00:04Title
  • 00:26Heat Stress
  • 02:46Ethanol Fixation and DAPI Staining
  • 04:10Acetone Fixation
  • 05:21Microscopic Imaging
  • 07:56Results: Visualization of C. elegans Exl-1 Subcellular Localization through GFP Tagging and DAPI Staining
  • 09:07Conclusion

Summary

Automatic Translation

Denne protokollen demonstrerer hvordan å spore et protein kjernefysiske translokasjon under varmestress med en grønn fluorescens protein (GFP) fusion protein som en markør og 4', 6-diamidino-2-phenylindole (DAPI) flekker. DAPI flekker protokollen er rask og beholder GFP og protein subcellular lokalisering signaler.

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