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JoVE Journal
Developmental Biology

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3D Cocultures 星形胶质细胞与人多潜能干细胞神经元的突触电路建模
 
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3D Cocultures 星形胶质细胞与人多潜能干细胞神经元的突触电路建模

Article DOI: 10.3791/58034-v 08:48 min August 16th, 2018
August 16th, 2018

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Summary

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在本协议中, 我们的目的是描述一种可重现的方法, 将分离的人类多能干细胞衍生神经元和星形胶质细胞组合成3D 球体 cocultures, 在自由漂浮条件下维持这些球体, 随后用 immunoanalysis 和多电极阵列记录测量球体的突触电路活动。

Tags

发育生物学 问题 138 干细胞 神经元 星形胶质细胞 突触 Organoid 共培养 生物反应器 多电极阵列
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