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3D Cocultures 星形胶质细胞与人多潜能干细胞神经元的突触电路建模
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
DOI:

08:48 min

August 16, 2018

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Chapters

  • 00:04Title
  • 00:49Generation and Maintenance of Astrocyte Progenitors (hAstros) from hPSCs
  • 02:41Generation and Maintenance of Neurons (hNeurons) and Inducible Neurons (iNeurons) from hPSCs
  • 03:27Preparation and Maintenance of 3D Sphere Co-cultures
  • 05:42Measurement of Live Synaptic Physiology with Multielectrode Arrays (MEAs)
  • 06:56Results: Morphological and Physiological Characteristics of Synaptic Microcircuits
  • 08:07Conclusion

Summary

Automatic Translation

在本协议中, 我们的目的是描述一种可重现的方法, 将分离的人类多能干细胞衍生神经元和星形胶质细胞组合成3D 球体 cocultures, 在自由漂浮条件下维持这些球体, 随后用 immunoanalysis 和多电极阵列记录测量球体的突触电路活动。

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