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Synaptic dataloger modellering med 3D Cocultures astrocytter og neuroner fra humane pluripotente stamceller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells
DOI:

08:48 min

August 16, 2018

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Chapters

  • 00:04Title
  • 00:49Generation and Maintenance of Astrocyte Progenitors (hAstros) from hPSCs
  • 02:41Generation and Maintenance of Neurons (hNeurons) and Inducible Neurons (iNeurons) from hPSCs
  • 03:27Preparation and Maintenance of 3D Sphere Co-cultures
  • 05:42Measurement of Live Synaptic Physiology with Multielectrode Arrays (MEAs)
  • 06:56Results: Morphological and Physiological Characteristics of Synaptic Microcircuits
  • 08:07Conclusion

Summary

Automatic Translation

I denne protokol, vi har til formål at beskrive en reproducerbar metode til at kombinere dissocieres humane pluripotente stamceller afledt neuroner og astrocytter sammen i 3D sfære cocultures, vedligeholdelse af disse områder i frie flydende betingelser, og efterfølgende måle synaptic kredsløb aktivitet af kugler med immunoanalysis og multielectrode array optagelser.

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