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用线 Myography 测定小鼠肠系膜动脉的等轴收缩力
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Medicine
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JoVE Journal
Medicine
Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography
Please note that all translations are automatically generated.
Click here for the English version.
用线 Myography 测定小鼠肠系膜动脉的等轴收缩力
DOI:
10.3791/58064-v
•
09:42 min
•
August 20, 2018
•
Jie Sun
,
Guang Ming Yang
,
Tao Tao
,
Li Sha Wei
,
Yang Pan
,
Min Sheng Zhu
1
Model Animal Research Center and MOE Key Laboratory of Model Animal and Disease Study
,
Nanjing University
,
2
School of Pharmacy
,
Nanjing University of Chinese Medicine
Chapters
00:04
Title
00:55
Mouse Mesenteric Artery Dissection
02:26
Arterial Mounting
05:30
Normalization
07:03
Artery Contraction Recording
08:18
Results: Representative Tracings Showing the Normalization Procedure, Potentiation by High-K+ Solution, and Contraction
08:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
采用线 myograph 技术对血管平滑肌功能和筛查新药进行了研究。我们报告一个详细的协议, 以测量的等距收缩性的小鼠肠系膜动脉和筛选新的松弛剂血管平滑肌。
Tags
Isometric Contractility
Wire Myography
Mesenteric Artery
Vascular Smooth Muscle
Contractility Measurement
Hypertension Therapy
High Potassium
Multi-channel System
Drug Screening
Euthanized Mouse
Gastrointestinal Tract
Mesenteric Artery Dissection
HEPES-Tyrode Solution
Stereoscopic Microscope
Calcium-free Solution
Artery Segment
Stainless Steel Wire
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