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原代培养小鼠肠嗜铬细胞的全细胞电生理
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Biology
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JoVE Journal Biology
Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells
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原代培养小鼠肠嗜铬细胞的全细胞电生理

DOI:
10.3791/58112-v

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10:04 min

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September 26, 2018

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, , , , ,
1Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering,Mayo Clinic, 2Division of Gastroenterology, Department of Medicine,University of Massachusetts Medical School

Chapters

  • 00:04Title
  • 00:45Tissue Isolation
  • 02:08Enzymatic and Mechanical Digestion
  • 04:20Cell Culture
  • 05:16Whole Cell Electrophysiology of EC Cells from Primary Culture
  • 08:06Results: Culture Results and Throughput of Whole-cell Electrophysiology
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

肠嗜铬 (EC) 细胞包括一个小的胃肠上皮细胞子集。ec 细胞是电兴奋和释放血清素, 但培养和鉴定 EC 细胞的困难是有限的生理学研究。本文提出的方法建立了一种能通过电生理学检查单个 EC 细胞的主要培养模型。

Tags

Whole Cell ElectrophysiologyPrimary Cultured Murine Enterochromaffin CellsEnteric NeuroscienceGastrointestinal PhysiologyEnterochromaffin Cell ExcitabilityGastrointestinal Hormone ReleaseSingle-cell TechniquesElectrophysiologyPBSMicrodissectionCollagenase Digestion

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