This content is Free Access.
1Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, 3Center for Nano Science,Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen
Chapters
- 00:04Title
- 00:39Immobilization of Proteins via Functional Silane Coupling Agents
- 04:42Atomic Force Microscopy Based Single Molecule Force Spectroscopy
- 08:38Results: Single Force Spectroscopy
- 10:07Conclusion
Denne protokollen beskriver kovalente immobilisering av proteiner med en heterobifunctional silane kopling agent silisium oksid overflater laget for atomic force mikroskopi basert ett molekyl force spektroskopi som er eksemplifisert ved den samspillet av RrgA (pilus-1 tips adhesin av S. pneumoniae) med fibronectin.
