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JoVE Journal
Biochemistry

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改进杆状病毒表达载体生产昆虫细胞分泌植物蛋白
 
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改进杆状病毒表达载体生产昆虫细胞分泌植物蛋白

Article DOI: 10.3791/58283 09:14 min
August 20th, 2018

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Summary August 20th, 2018

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在这里, 我们提出了利用昆虫细胞和杆状病毒蛋白表达系统来产生大量植物分泌蛋白的蛋白质结晶的协议。用 GP67 或昆虫 hemolin 信号肽对昆虫细胞中植物蛋白分泌表达进行了改进, 并对杆状病毒表达载体进行了修正。

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