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Journal
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Immunology and Infection
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乙型肝炎病毒感染原代肝细胞和库珀细胞的 "肝片上" 培养
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
Please note that all translations are automatically generated.
Click here for the English version.
乙型肝炎病毒感染原代肝细胞和库珀细胞的 "肝片上" 培养
DOI:
10.3791/58333-v
•
10:25 min
•
February 19, 2019
•
Ana Maria Ortega-Prieto
,
Jessica Katy Skelton
,
Catherine Cherry
,
Marco Antonio Briones-Orta
,
Charlotte Alexandra Hateley
,
Marcus Dorner
1
Section of Virology, Department of Medicine
,
Imperial College
Chapters
00:04
Title
01:38
Assembly and Equilibration of Plates
04:36
Thawing and Seeding of Hepatocytes for Monocultures
07:38
Results: Long-term Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
09:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目标是提供一个分步指南, 以执行三维 "片上的肝脏上" 感染实验与乙型肝炎病毒。
Tags
Liver-on-a-chip
Primary Hepatocytes
Kupffer Cells
Hepatitis B Virus
Viral Pathogenesis
Infection Kinetics
Viral Replication
Immune Invasion
Physiological Model
Drug Testing
Hepatic Microenvironment
Hepatotropic Infections
Liver Diseases
Drug Metabolism
Long-term Culture
Specialized Tools
Visual Demonstration
Membrane
Microfluidic Plates
Seeding
Medium Priming
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