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Immunology and Infection

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"Lever-on-a-Chip" kulturer af primære hepatocytter og Kupffer celler for Hepatitis B virusinfektion

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

"Lever-on-a-Chip" kulturer af primære hepatocytter og Kupffer celler for Hepatitis B virusinfektion

DOI:

10.3791/58333-v

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10:25 min

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February 19, 2019

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Ana Maria Ortega-Prieto, Jessica Katy Skelton, Catherine Cherry, Marco Antonio Briones-Orta, Charlotte Alexandra Hateley, Marcus Dorner

¹Section of Virology, Department of Medicine,Imperial College

Chapters

00:04Title
01:38Assembly and Equilibration of Plates
04:36Thawing and Seeding of Hepatocytes for Monocultures
07:38Results: Long-term Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
09:13Conclusion

Summary

Automatic Translation

Målet med denne protokol er at give en trin for trin guide til at udføre 3-D "leveren-on-a-chip" infektion eksperimenter med hepatitis B-virus.

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