/

/

体内新生小鼠皮层神经元的双光子成像

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

Please note that all translations are automatically generated. Click here for the English version.

体内新生小鼠皮层神经元的双光子成像

DOI:

10.3791/58340-v

•

06:24 min

•

October 18, 2018

•

Hidenobu Mizuno^2,3, Shingo Nakazawa³, Takuji Iwasato³

¹International Research Center for Medical Sciences (IRCMS),Kumamoto University, ²Division of Neurogenetics,National Institute of Genetics, ³Department of Genetics,SOKENDAI (The Graduate University for Advanced Studies)

Chapters

00:04Title
00:33Exposing the Skull
01:29Cranial Window Preparation
03:29Two-Photon Imaging
04:59Results: In vivo Two-Photon Imaging of Cortical Neurons in Neonatal Mice
05:57Conclusion

Summary

Automatic Translation

我们提出了一种用于成像新生小鼠大脑皮层的活体双光子成像协议。该方法适用于分析皮层神经元的发育动态、控制神经元动力学的分子机制以及疾病模型神经元动力学的变化。

Tags

In Vivo Two-photon Imaging Cortical Neurons Neonatal Mice Neuroscience Neuron Morphology Cranial Window Anesthesia Craniotomy Agarose Gel Cover Slip Dental Cement Titanium Bar Analgesic

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

一个慢性双光子薄颅骨窗口技术在体内 Neuroinflammation模型小鼠小胶质细胞成像

在体内脊髓的鼠标线使用双光子显微镜成像

在体内双光子成像的经验依赖的分子在大脑皮质神经元的变化

在皮肤中的单神经末梢体内双光子显微镜

双光子<em>在体内</em>树突棘中的鼠标皮质用稀疏的头颅成像准备

细胞动力学在小鼠脊髓双光子成像

同时双光子<em>在体内</em>突触输入的影像和突触后目标中的鼠标Retrosplenial的Cortex

大鼠皮层神经元丝状肌动蛋白（F-肌动蛋白）泪点的量化

脑切片神经元树突的双光子钙成像

可视化蛋白质激酶在头部固定行为小鼠中使用 Vivo 双光子荧光寿命成像显微镜的活性

Read Article