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In Vivo To-foton billeddannelse af kortikale neuroner i Neonatal mus
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JoVE Journal Neuroscience
In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

In Vivo To-foton billeddannelse af kortikale neuroner i Neonatal mus

DOI:
10.3791/58340-v

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06:24 min

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October 18, 2018

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, ,
1International Research Center for Medical Sciences (IRCMS),Kumamoto University, 2Division of Neurogenetics,National Institute of Genetics, 3Department of Genetics,SOKENDAI (The Graduate University for Advanced Studies)

Chapters

  • 00:04Title
  • 00:33Exposing the Skull
  • 01:29Cranial Window Preparation
  • 03:29Two-Photon Imaging
  • 04:59Results: In vivo Two-Photon Imaging of Cortical Neurons in Neonatal Mice
  • 05:57Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en i vivo to-foton imaging protokol for imaging hjernebarken af neonatal mus. Denne metode er egnet til at analysere den udviklingsmæssige dynamics af kortikale neuroner, de molekylære mekanismer, der styrer neuronal dynamik og ændringer i neuronal dynamics i sygdomsmodeller.

Tags

In VivoTwo-photon ImagingCortical NeuronsNeonatal MiceNeuroscienceNeuron MorphologyCranial WindowAnesthesiaCraniotomyAgarose GelCover SlipDental CementTitanium BarAnalgesic

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