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Journal
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Medicine
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用于疾病建模的人三维肺组织培养 (3D-LTCs) 的生成
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
Please note that all translations are automatically generated.
Click here for the English version.
用于疾病建模的人三维肺组织培养 (3D-LTCs) 的生成
DOI:
10.3791/58437-v
•
05:47 min
•
February 12, 2019
•
Michael Gerckens
2,3
,
Hani N. Alsafadi
5,6
,
Darcy E. Wagner
5,6
,
Michael Lindner
7
,
Gerald Burgstaller*
1,2,3
,
Melanie Königshoff*
1,2,3,8
1
Comprehensive Pneumology Center
,
Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich
,
2
German Center of Lung Research (DZL)
,
3
Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München
,
Comprehensive Pneumology Center Munich DZL/CPC-M
,
4
Department of Experimental Medical Science, Lung Bioengineering and Regeneration
,
Lund University
,
5
Wallenberg Center for Molecular Medicine
,
Lund University
,
6
Stem Cell Centre
,
Lund University
,
7
Asklepios Fachkliniken Munich-Gauting
,
8
Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine
,
University of Colorado
Chapters
00:04
Title
00:49
Lung Tissue Inflation
02:33
Precision-cut Lung Slicing (PCLS) and PCLS Punch Generation
04:02
Results: Representative Experimental 48 h Human 3D-lung Tissue Culture Readouts
04:55
Conclusion
Summary
Automatic Translation
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在这里, 我们提出了一个方案, 用于制备富含琼脂的人精确切肺切片从切除的患者组织, 适合产生三维肺组织培养, 以模拟人类肺部疾病的生物和生物医学研究。
Tags
3D-LTCs
Disease Modeling
Ex Vivo
Agarose
Bronchus
Canula
Precision Cut Lung Slicing
Vibratome
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