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Journal
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Immunology and Infection
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新生儿多药脓毒症的对照小鼠模型
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
A Controlled Mouse Model for Neonatal Polymicrobial Sepsis
Please note that all translations are automatically generated.
Click here for the English version.
新生儿多药脓毒症的对照小鼠模型
DOI:
10.3791/58574-v
•
14:54 min
•
January 27, 2019
•
Byron Brook*
1
,
Nelly Amenyogbe*
1
,
Rym Ben-Othman
,
Bing Cai
,
Danny Harbeson
,
Freddy Francis
,
Aaron C. Liu
,
Natallia Varankovich
,
James Wynn
4
,
Tobias R. Kollmann
2
1
Department of Experimental Medicine
,
University of British Columbia
,
2
Department of Pediatrics, Division of Infectious Diseases
,
University of British Columbia
,
3
Department of Pediatrics, College of Medicine
,
University of Florida
,
4
Department of Pathology, Immunology, and Laboratory Medicine
,
University of Florida
Chapters
00:00
Title
00:33
Preparation of Frozen Cecal Slurry Stock from Adult Mouse Donors
04:42
Challenging Newborn Mice with a Weight-adjusted Dose of Cecal Slurry
08:00
Monitoring Mice and Determining Humane Endpoint
10:34
Representative Results
14:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案为建立和评估7天龄小鼠新生儿败血症提供了必要的步骤。
Tags
Neonatal Polymicrobial Sepsis
Cecal Slurry Model
Standardized Procedure
Data-driven Approach
Moribund Pups
Frozen Cecal Slurry Stock
Cecal Contents
Dissection
Sterile Tools
Biological Safety Cabinet
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