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Immunology and Infection
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Fordøjelsen af Murine leveren en Flow Cytometric analyse af lymfatisk Endothelial Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Fordøjelsen af Murine leveren en Flow Cytometric analyse af lymfatisk Endothelial Cells

DOI:
10.3791/58621-v

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08:07 min

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January 07, 2019

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, ,
1Division of Gastroenterology and Hepatology, Department of Medicine,University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology,University of Colorado Anschutz Medical Campus

Chapters

  • 00:04Title
  • 00:52Liver Single-cell Suspension Preparation
  • 03:36Flow Cytometric Analysis
  • 05:49Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at identificere lymfe endotel cellepopulationer i leveren ved hjælp af beskrevet markører. Vi udnytte collagenase IV og DNase og en blid hakning af væv, kombineret med flowcytometri, for at identificere en særskilt population af lymfatisk endotelceller.

Tags

LiverLymphatic Endothelial CellsFlow CytometrySingle-cell SuspensionLiver Biology

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