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Fordøjelsen af Murine leveren en Flow Cytometric analyse af lymfatisk Endothelial Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells
DOI:

08:07 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:52Liver Single-cell Suspension Preparation
  • 03:36Flow Cytometric Analysis
  • 05:49Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization
  • 07:22Conclusion

Summary

Automatic Translation

Målet med denne protokol er at identificere lymfe endotel cellepopulationer i leveren ved hjælp af beskrevet markører. Vi udnytte collagenase IV og DNase og en blid hakning af væv, kombineret med flowcytometri, for at identificere en særskilt population af lymfatisk endotelceller.

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