9,440 Views
•
12:42 min
January 07, 2019
DOI:
10.3791/58651-v
ويصف هذا البروتوكول سير عمل مفصلة لتوليد والسابقين فيفو توصيف الفيروسات أونكوليتيك للتعبير عن إيمونومودولاتورس، باستخدام فيروسات الحصبة ترميز engagers خلية T بيسبيسيفيك كمثال. التطبيق والتكيف مع سائر المنابر ناقلات والمتسلسلات سيتم التعجيل بتنمية إيمونوفيروثيرابيوتيكس الرواية للترجمة السريرية.
07:20
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
Related Videos
14694 Views
08:52
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
17171 Views
08:51
Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
9030 Views
06:16
Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
12645 Views
07:36
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
6446 Views
08:02
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
2518 Views
09:15
Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
3351 Views
06:13
Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses
9297 Views
10:13
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
8936 Views
09:03
Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
8633 Views
Read Article
Cite this Article
Heidbuechel, J. P., Engeland, C. E. Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo. J. Vis. Exp. (143), e58651, doi:10.3791/58651 (2019).
Download .ris file
Copy
Share Video
.