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聚丙烯酰胺凝胶中蛋白质的荧光银染色
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
DOI:

06:24 min

April 21, 2019

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Chapters

  • 00:04Title
  • 00:36Preparation of the Gel
  • 01:08Fixation of the Gel
  • 01:40Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
  • 02:41Fluorogenic Development of the Gel
  • 04:05Destaining and Imaging
  • 04:34Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
  • 05:56Conclusion

Summary

Automatic Translation

在这里, 我们描述了一个详细的协议, 概述了一种新的荧光染色技术的总蛋白检测在聚丙烯酰胺凝胶。该协议采用了银离子特异性荧光开启探针, 可检测银+蛋白复合物, 并消除了传统的铬性银渍的某些限制。

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