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Journal
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Biochemistry
/
聚丙烯酰胺凝胶中蛋白质的荧光银染色
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Please note that all translations are automatically generated.
Click here for the English version.
聚丙烯酰胺凝胶中蛋白质的荧光银染色
DOI:
10.3791/58669-v
•
06:24 min
•
April 21, 2019
•
Alex Y. H. Wong
,
Sheng Xie
,
Ben Zhong Tang
,
Sijie Chen
1
Ming Wai Lau Centre for Reparative Medicine
,
Karolinska Institutet
,
2
Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science
,
Hong Kong University of Science and Technology
Chapters
00:04
Title
00:36
Preparation of the Gel
01:08
Fixation of the Gel
01:40
Preparation of the AgNO
3
Solution and Silver Impregnation of the Gel
02:41
Fluorogenic Development of the Gel
04:05
Destaining and Imaging
04:34
Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
05:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一个详细的协议, 概述了一种新的荧光染色技术的总蛋白检测在聚丙烯酰胺凝胶。该协议采用了银离子特异性荧光开启探针, 可检测银
+
蛋白复合物, 并消除了传统的铬性银渍的某些限制。
Tags
Fluorescent Silver Staining
Polyacrylamide Gels
SDS-PAGE
Protein Visualization
TPE-4TA Dye
Silver Nitrate
Gel Electrophoresis
Fluorogenic Staining
Article
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