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1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Chapters
- 00:04Title
- 00:36Preparation of the Gel
- 01:08Fixation of the Gel
- 01:40Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
- 02:41Fluorogenic Development of the Gel
- 04:05Destaining and Imaging
- 04:34Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
- 05:56Conclusion
Hier beschreiben wir ein detailliertes Protokoll umreißt eine neue fluoreszierende Färbung Technik für Gesamt-Protein Nachweis in Polyacrylamid-Gele. Das Protokoll nutzt eine Sonde, Silber Ionen-spezifische Fluoreszenz Turn-on, die Ag+erkennt-Proteinkomplexe, und gewisse Einschränkungen der traditionellen chromogenen Silber Flecken beseitigt.
